MOUSE METHOD ::
Mice show characteristic convulsions after s.c. inj. of insulin at elevated temperatures The percentage convulsions produced by the test and standard Preparations are compared
Experimental procedure ::
Minimum 100 mice weighing between 18-22 gms of the same strain are used They Should be maintained on constant diet They should be fasted 18 hrs prior to the experiment
Standard and sample dilutions ::
Dilutions are prepared with sterile saline solution so as to contain 0.064 units/ml (std dilution I) and 0.096 untis/ml (std.dilution II) Similarly test sample solutions are also prepared
Mice are divided into 4 groups each containing 25 mice and insulin is injected s.c. as follows :
100 MICE
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25 25 25 25
Standard Standard Test Sample Test Sample
Dilution Dilution Dilution Dilution
(0.064 units/ml) (0.096 units/ml)
Mice are put in an air incubator at 33.C and observed for one and a half hr An air incubater with a glass front provided with six shelves is used The temperature is thermostatically controlled Two mice are kept in each of the boxes made up of perforated sheets of metal
The mice which convulse or die are taken out of the incubator and observed These mice usually convulse severely but failure of the animal to upright it self when placed on its back should as well be considered as convulsion Convulsive mice may be saved by an inj of 0.5 ml. of 5% dextrose solution
Percentage convulsions produced by the test sample are compared with those of the standard sample
Those animals which survive may be used again for another expt after an interval of one week
Dose S10.25 ml S20.25 ml T1 0.25 ml T2 0.25 ml
Convulsions
Percentage
Convulsions
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