Experimental Procedure ::
Animals are divided into 4 groups of 3 rabbits each The rabbits are then put into an animal holder They should be handled with care to avoid excitement
First part of the test ::
A sample of blood is taken from the marginal ear vein of each rabbit Presence of reducing sugar is estimated per 100 ml of blood by a suitable chemical method This concentration is called Initial Blood sugar Level
The four groups of rabbits are then given sc injections of insulin as follows
12 RABBITS
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3 3 3 3
Standard Standard Test Sample Test Sample
Dilution Dilution Dilution Dilution
(I) (II) (I) (II)
From each rabbit a sample of blood is withdrawn upto 5 hrs at the interval of 1 hr each Blood sugar is determined again This is known as Final Blood Sugar Level
Second part of the test ::
The same animals are used for the second part The experiment can be carried out after one week Again they are fasted and initial blood sugar is determined The grouping is reversed that is to say those animals which received the standard are given the test and those which received the test are now given the standard Those animals which received the less dose of the standard are given the higher dose of the test sample and vice versa This test is known as Twin Cross Over Test
Mean percentage decrease in blood sugar of the first and second part is calculated
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