METHOD USING RABBITS DUODENUM ::
A rabbit weighing 2-3 kg is killed by headblow method and bled to death The animal is dissected out the abdominal organs are exposed to isolate the duodenum The duodenum is suspended in the inner
bath of the mammalian organ bath Inner bath is filled with Tyrode solution which is maintained at 37.5.C The muscle is allowed to stabilize for 30 min Rhythmic contractions are recorded by means of the isotonic frontal writing lever on the kymograph Adrenaline relaxes the duodenum and the same property is taken upto consideration for finding out the potency of the test sample Adrenaline is bioassayed by graphical method as shown in
De Jalon s Method on Rat s Uterus ::
This method was first demonstrated by De Jalon . A virgin female rat weighing about 100-150 g is used for the experiment The rat is killed by the headblow method and bled to death The abdomen is opened and uterine horns are isolated and placed in a petri dish containing de Jalon solution Uterine horns are ligated and suspended in a mammalian organ bath containing modified Ringer solution having the following composition is used NaCI 9.0 gm CaCI2 0.06 gm KCI 0.45 gm Dextrose 0.5 gm NaHCO3 0.5 gm and distilled water 1000 ml This solution abolishes the rhythmic contraction of the uterus It is maintained at 37.C and bubbled continuously with air
Two identical contractions for 30 sec with carbachol are recorded (0.75 mcg/ml) Then selecting three different doses of standard adrenaline and one intermediate dose test adrenaline the percentage reduction of carbachol induced contractions are recorded
Straub s Method ::
In this method a glass cannula is passed through the aorta and valves into the ventricle of heart so that the solution is pumped up and down in the cannula with every beat A graph paper attached behind the cannula to give an estimate of the force of contraction of systole of the heart at every beat The beat is recorded by attaching the tip of the ventricle through a thread to a lever This preparation has been
widely use for the detection of small quantity of adrenaline Adrenaline increases the force of contraction of the ventricle The increased force produced by the test sample is compared with that of the standard preparation
0 Comments